A COMPOSITE, IN-HOUSE ENZYME IMMUNOASSAY FOR DETECTION OF ROTAVIRUS AND ADENOVIRUS IN FAECAL SAMPLES.

T.W. Kok, A. Sharley, L. Payne, R.B. Johnson and J. Pacyna
Australian Journal of Medical Science Volume19, Number 2, May 1998

ABSTRACT:

A number of commercially available enzyme immunoassays (EIA) require the use of two separate assays for detection of rotavirus and adenovirus, using antisera produced in-house from purified viruses, we develop a composite microwell EIA for detection of rotavirus and adenovirus in faecal samples. These antisera were tested and validated with reference reagents and commercially available antisera. The limit of detection for rotavirus was 2.5 x 103 viral particles/ml and for adenovirus was 1 x 103/ml in this composite EIA. During the period January 1996 to July 1197, 3,618 samples were tested, of which 371 (10.3%) were positive for rotavirus, 169 (4.7%) adenovirus positive and 3 (0.08%) were positive for both rotavirus and adenovirus. The composite EIA is more efficient as it requires less reagents, materials and labour then using two separate assays.